Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology
نویسندگان
چکیده
منابع مشابه
Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology.
Quantitative PCR (Q-PCR or real-time PCR) approaches are now widely applied in microbial ecology to quantify the abundance and expression of taxonomic and functional gene markers within the environment. Q-PCR-based analyses combine 'traditional' end-point detection PCR with fluorescent detection technologies to record the accumulation of amplicons in 'real time' during each cycle of the PCR amp...
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I n the last few years, molecular hybridization methods have been used extensively in basic and applied virology because of their technical flexibility and high specificity. Using these techniques, the detection of DNA and RNA viruses directly from clinical specimens, the analysis of the specific transcriptional activity of viral genes in vitro and in vivo, and the study of virus-host relations...
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Two different quantitative PCR platforms, droplet digital PCR (dd-PCR) and quantitative real-time PCR (qPCR), were compared in a mcrA-based methanogen community assay that quantifies ten methanogen sub-groups. Both technologies exhibited similar PCR efficiencies over at least four orders of magnitude and the same lower limits of detection (8 copies μL-DNA extract-1). The mcrA-based methanogen c...
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ژورنال
عنوان ژورنال: FEMS Microbiology Ecology
سال: 2009
ISSN: 0168-6496,1574-6941
DOI: 10.1111/j.1574-6941.2008.00629.x